Which of the following is NOT recommended to be done before drawing blood for an i-STAT?

Pumping the fist multiple times before drawing blood is not recommended because it can cause hemoconcentration, which significantly alters the concentration of various components in the blood. This can lead to inaccurate test results, making it challenging to interpret the patient's condition effectively.

In contrast, cleaning the site with an antiseptic is essential for preventing contamination and ensuring the integrity of the blood sample. Warming the extremity can help improve blood flow, making it easier to obtain a sample. Using a tourniquet is a standard practice that helps engorge the veins, making them more prominent for easier access. All of these actions, apart from pumping the fist, contribute to obtaining an accurate and reliable blood sample necessary for the i-STAT testing process.